Fig. 3

The dsDNA@DMONs trigger the activation of type I interferon signaling and promote the maturation and antigen presentation ability of DC cells. A RT-qPCR analysis of the gene expression of Ifnb, Cxcl10, and Isg15 in DC2.4 cells treated with PBS (Control, Ctrl), DMONs (Si: 6 μg/mL), dsDNA (2.4 μg/mL) or dsDNA@DMONs (Si: 6 μg/mL) for 24 h. B Western blot of p-TBK1 in DC2.4 cells treated with PBS, DMONs (Si: 6 μg/mL), dsDNA (2.4 μg/mL) or dsDNA@DMONs (Si: 6 μg/mL) for 24 h. GAPDH serves as a loading control. C–E Mean fluorescent intensity (MFI) of C CD80 and D CD86 by FACS analysis in DC2.4 cells treated with PBS, DMONs (Si: 6 μg/mL), dsDNA (2.4 μg/mL) or dsDNA@DMONs (Si: 6 μg/mL) for 24 h, scatter plots of CD80 and CD86 were shown in E. F RT-qPCR analysis of the gene expression of B2m, Psmb5, Tap1, Tap2, and Tapbp in DC2.4 cells treated with PBS, DMONs (Si: 6 μg/mL), dsDNA (2.4 μg/mL) or dsDNA@DMONs (Si: 6 μg/mL) for 24 h. Data are shown as mean ± SEM (n ≥ 3). P value was calculated by unpaired Student's t-test in A, C, D and F. (**p < 0.01; ***p < 0.001)