Fig. 6

Deposition of proteins of the human complement system on PNPs. The adsorbed proteins were analyzed by LC-MS/MS and Western blot (WB) analyses. A. Adsorbed immunoglobulins such as IgG (i.e., IgG1, IgG2, IgG3) and IgM were detected by LC-MS/MS. The deposition of human IgG (B) and IgM (C) was also analyzed by WB analysis (attended band: 50 kDa and 75 kDa, respectively). Components of the classical complement activation pathway were analyzed by LC-MS/MS analysis (D) and the bands corresponding to C1q chains were visualized by WB (E, attended bands of C1q at ~ 23, 27 and 29 kDa). Proteins of the alternative (F) pathway were detected by LC-MS/MS analysis. LC-MS/MS data are shown as boxplots with median value. * p-value < 0.05; ** p-value < 0.01. uPNPs: untargeted nanoparticles; tPNPs: targeted nanoparticles; HS: human serum; HP: human plasma; empty: empty well