Fig. 7

Deposition of human common pathway complement proteins and inhibitors on PNPs. The adsorbed proteins were analyzed by LC-MS/MS and Western blot (WB) analyses. The deposition of proteins of the common pathway of complement activation (C3, C5, C6, C7, C8 and C9) was quantified by LC-MS/MS (A). To better evaluate complement activation, C3 fragmentation was analyzed by WB (B, C3ß: 63 kDa; C3α’2: 40 kDa). WB was also used to visualize the deposition of C6 (C, attended band at ~ 100 kDa), C7 (D, attended band at ~ 95 kDa) and C9 (E, attended band at ~ 70 kDa). Complement inhibitors were quantified by LC-MS/MS analysis (F). uPNPs: untargeted nanoparticles; tPNPs: targeted nanoparticles; HS: human serum; HP: human plasma; empty: empty well; C1 inh: C1 inhibitor; C4BP: C4 binding protein