Fig. 6

Panx1-targeting nanobodies reduce liver histopathology in acetaminophen-overdosed mice. Adult mice were overdosed with acetaminophen (APAP) (300 mg/kg) or kept untreated (UTC). After 2 h, some mice were additionally administered either nanobody (Nb1, Nb3, Nb9 or non-targeting Nb (10 mg/kg)) or N-acetylcysteine (NAC) (200 mg/kg). Sampling was performed 24 h after APAP overdosing. a Liver sections (10 µm) were stained with hematoxylin and periodic acid Schiff base (H-PAS) and b liver histopathology was evaluated using Suzuki’s score-method quantifying for congestion, vacuolization and necrosis (n = 4 (UTC and APAP) or n = 12 (Nb1, Nb3, Nb9, non-targeting Nb and NAC) animals per group). All data was analysed by parametric 1-way analysis of variance followed by post hoc tests with Bonferroni’s correction. Data were expressed as means ± S.D