Table 1 Overview antibodies

In vitro characterisation of DUBCA Panx1 cells (immunoblotting)

Clone D9M1C (Panx1) (Cell Signaling Technology)

1/500 in blocking buffer

24 h

4 °C

Goat anti-rabbit horseradish peroxidase-conjugated antibody (Clone P0448) (Dako)

1/500 in blocking buffer

1 h

Room temperature

In vitro characterisation of DUBCA Panx1 cells (immunocytochemistry)

Clone ABN242 (Panx1) (Merck)

1/250 in permeabilization buffer

24 h

4 °C

Donkey anti-rabbit Alexa Fluor^® 594-conjugated antibody (Clone A-21207) (Thermo Fisher Scientific)

1/250 in permeabilization buffer

1 h

Room temperature

Flow cytometry with Panx1-targeting nanobodies

Hemagglutinin Alexa Fluor^® 488-conjugated antibody (Clone 901509) (BioLegend)

1/1000 in flow cytometry buffer

20 min

4 °C

In vitro inflammation assay

Clone ab254360 (IL-1β) (Abcam)

1/500 in blocking buffer

24 h

4 °C

Goat anti-rabbit Alexa Fluor^® 488-conjugated antibody (Clone ab150077) (Abcam)

1/500 in blocking buffer

1.5 h

4 °C

Immunoblot analysis of liver proteins

Clone sc-514 (caspase-1) (Santa Cruz)

1/500 in blocking buffer

24 h

4 °C

Clone HPA009128 (CYP2E1) (Atlas Antibodies)

1/500 in blocking buffer

24 h

4 °C

Clone ab254360 (IL-1β) (Abcam)

1/500 in blocking buffer

24 h

4 °C

Clone ab263899 (NLRP3) (Abcam)

1/500 in blocking buffer

24 h

4 °C

Clone D9M1C (Panx1) (Cell Signaling Technology)

1/500 in blocking buffer

24 h

4 °C

Goat anti-mouse horseradish peroxidase-conjugated antibody (Clone P0447) (Dako, USA)

1/1000 in blocking buffer

1 h

Room temperature

Goat anti-rabbit horseradish peroxidase-conjugated antibody (Clone P0448) (Dako, USA)

1/1000 in blocking buffer

1 h

Room temperature