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Table 3 Equilibrium dissociation constants of Panx1-targeting nanobodies

From: Nanobody-based pannexin1 channel inhibitors reduce inflammation in acute liver injury

Equilibrium dissociation constants (Kd) of Panx1-targeting nanobodies

Nanobody

mPanx1

hPanx1

Nb1

6.6 ± 3.0 nM

6.9 ± 2.9 nM

Nb3

2.4 ± 1.5 nM

0.7 ± 2.5 nM

Nb9

206.6 ± 71.7 nM

277.4 ± 67.2 nM

  1. Affinity for mouse Panx1 (mPanx1) and human Panx1 (hPanx1) was evaluated by incubating DUBCA WT, DUBCA mPanx1 and DUBCA hPanx1 cells with individual Panx1 nanobody clones in different concentrations ranging from 0 up to 600 nM. Recorded binding values were used to define equilibrium dissociation constant (Kd) values
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Journal of Nanobiotechnology

ISSN: 1477-3155

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