Fig. 6

In vitro therapeutic effect of FeOOH&mSiO₂ Janus nanoparticles conducted with 4T1 breast cancer cells. (A) Cell viability of 4T1 cells incubated with FMS/FMS-DOX/C-FMS under different conditions. (B) Confocal laser scanning microscopy (CLSM) images of DCFH-DA stained 4T1 cells after incubation with fresh medium and FMS with or without H₂O₂ and GSH after 8 h. (C) CLSM images of Calcein AM (green, live cells) and PI (red, dead cells) co-stained 4T1 cells after incubation with fresh medium, FMS/FMS-DOX with or without H₂O₂ and GSH for 12 h. (D) CLSM images of 4T1 cells stained with DAPI after incubation with FMS-DOX at different time points. (E) Flow cytometric quantitative analysis of Annexin V-FITC/PI co-stained 4T1 cells after co-incubation with FMS/FMS-DOX with or without H₂O₂ and GSH under weak acidic (pH 5.4) conditions for 12 h. The dosage of FMS and FMS-DOX in the in vitro experiments was all controlled at 200 µg/mL. The concentration of H₂O₂ and GSH in the in vitro experiments was all controlled at 10 mM. Scale bar: 100 μm