Fig. 3

Biocompatibility and chemokine sequestration of hydrogels. A, B, C) CCK-8 assay of HUVECs, NIH 3T3, and RAW264.7 cells co-cultured with PBS or hydrogels after 24 h (n = 3). D) Confocal microscopy images of live/dead staining assay of Raw264.7 cells co-cultured with PBS or hydrogels after 24 h. Scale bar: 200 μm. E, F, G) MCP-1, VEGF, and IL-8 bound amount to hydrogels. One hundred nanograms of MCP-1, VEGF, and IL-8 was added to 50 µl different hydrogels and sequestration was determined after co-incubation with hydrogels for 24 h. H) Hemolysis photographs and ratio (%) after exposure to hydrogels. I) cumulative release of PBNPs from PB@Algs-H hydrogels. (n = 3; mean ± s.d.; ns, not statistically significant; **p < 0.01, ***p < 0.001, ****p < 0.0001)