Fig. 7

Anti-inflammatory effects of hydrogels on deep second-degree burn wounds. A) Flow cytometry analysis indicating the proportion of M2 macrophages (CD11b⁺CD206⁺) on day 7. B, C) Statistical data of the percentage of Arg-1⁺ and iNOS⁺ macrophages. D) Representative immunofluorescence staining images of iNOS and Arg-1 on day 7. Scale bar: 100 μm. E) DHE staining images indicating ROS levels in wound tissue on day 7. Scale bar: 100 μm. G, H) Concentrations of TNF-α and IL-6 in wound tissue measured by ELISA on day 4. (n = 3; mean ± s.d.; *p < 0.05, **p < 0.01, ***p < 0.001)