Fig. 4
A Viability of B16F10 cells treated with different preparations for 48 h. C (ng/mL) indicates the concentration of TPL in each preparation. B IC50 values of different preparations. C Determination of Fe2+ content after different treatments. D Degradation of MB in the presence of H2O2 at pH 5.5 and 7.4. E ROS levels in B16F10 cells detected by flow cytometry after different treatments. F Quantification of the results from (E). Data are expressed as the mean ± SD (n = 4). ****P < 0.0001. G Fluorescence imaging of ROS in B16F10 cells treated with different preparations. Scale bar:50 μm