Fig. 5

In vitro biocompatibility of membranes. A Immunofluorescence staining images (blue, nuclei stained with DAPI; green, cellular F-actin stained with FITC-phalloidin) of D1 cells on the different membranes on day 7. B SEM images of D1 cells on the surfaces of the different membranes on day 7. Red arrow: cells. C Live/dead staining of D1 cells expanded on the different membranes on day 7 (red, dead cells; green, live cells). D CCK-8 assay to determine the proliferation of D1 cells on the different membranes on days 1, 3, and 7 (n = 3). E Images of EdU staining of D1 cells on the different membranes on day 7 (red, EdU⁺ cells; blue, cell nuclei). F Semiquantitative analysis of EdU⁺ staining of cells. *** p < 0.01; **** p < 0.001 vs the PG membrane; ^### p < 0.01; ^#### p < 0.001 vs the PGH membrane