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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Biomineralized tetramethylpyrazine-loaded PCL/gelatin nanofibrous membrane promotes vascularization and bone regeneration of rat cranium defects

Fig. 6

Osteogenic differentiation of D1 cells on the different membranes. A Expression of osteogenic marker genes: (a1) Runx2, (a2) Osterix, (a3) ALP, (a4) OCN, and (a5) COL1. B ALP activity staining of D1 cells on day 7. C Immunofluorescent staining of Runx2 expression on day 7 (blue, cell nucleus; red, Runx2). D ARS staining of D1 cells on day 21. ****p < 0.001; *****p < 0.0001 vs the PG membrane; ###p < 0.001. ####p < 0.0001 vs the PG membrane and the PGH membrane; &&&p < 0.001. &&&&p < 0.0001 vs day 3

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