Table 1 Real-time qPCR primer sequences
Gene | Primer | Primer sequence |
|---|---|---|
GAPDH | Forward | 5′—TTCACCACCTTCTTGATGTC-3ʹ |
Reverse | 5′—CACCACCAACTGCTTAGC-3ʹ | |
ALP | Forward | 5′—ATGGCCTGGTCCATCTCCAC-3ʹ |
Reverse | 5′—GCAGTATGAATTGAATCGGAACAAC-3ʹ | |
OPN | Forward | 5′—TATAGGATCTGGGTGCAGGCTGTA-3ʹ |
Reverse | 5′—TACGACCATGAGATTGGCAGTGA-3ʹ | |
Runx-2 | Forward | 5′—GGCTCACGTCGCTCATCTT-3ʹ |
Reverse | 5′—CTGCAAGCAGTATTTACAACAGAGG-3ʹ | |
Osterix | Forward | 5′—GCCAGATGGAAGCTGTGAAGA-3ʹ |
Reverse | 5′—AGGCCTTTGCCAGTGCCTA-3ʹ |