Table 2 Real-time qPCR primer sequences
Gene | Primer | Primer sequence |
|---|---|---|
GAPDH | Forward | 5′—CACCACCAACTGCTTAGC-3ʹ |
Reverse | 5′—TTCACCACCTTCTTGATGTC-3ʹ | |
HIF 1A | Forward | 5′—GAACGTCGAAAAGAAAAGTCTCG-3ʹ |
Reverse | 5′—CCTTATCAAGATGCGAACTCACA-3ʹ | |
FGF | Forward | 5′—GAGCGACCCACACGTCAAACTAC-3ʹ |
Reverse | 5′—CAGCCGTCCATCTTCCTTCATAGC-3ʹ | |
CD31 | Forward | 5′—AGCCAGCAGTATGAGGACCAGTC-3ʹ |
Reverse | 5′—TCCAATGACAACCACCGCAATGAG-3ʹ |