Fig. 2

Feasibility and electrode characterization of UCNPs-Cas14a based PEC. A) PAGE electropherograms to verify complementary strand triggered SDA of T2 aptamers (M1: 20 bp marker; 1: T-DNA; 2: cDNA; 3: cDNA + T-DNA; 4: cDNA + T-DNA + Nt; 6/7/8: 0.5/2/5 µM cDNA + T-DNA + KF + Nt; M2: 50 bp marker). DNA + KF; 5: cDNA + T-DNA + Nt; 6/7/8: 0.5/2/5 µM cDNA + T-DNA + KF + Nt; M2: 50 bp marker). B) AC impedance spectrum of the modified electrode [5 Mm Fe(CN)63-/4-(1:1), 0.1 M KCl, 100 KHZ-0.1 HZ]. C) Feasibility validation of UCNPs-Cas14a based PEC. D) SEM of CdS/ITO. E) SEM of CdS@Au/ITO. F) SEM of UCNPs-ssDNA-CdS@Au/ITO. G) XPS spectra of modified electrodes. XPS spectra of modified electrodes of Cd (H), S (I), Er (J), Au (K) and Yb (M)