Fig. 5
From: A universal CRISPR-Cas14a responsive triple-sensitized upconversion photoelectrochemical sensor
UCNPs-Cas14a based PEC for PTK7 detection. A) Schematic illustration of UCNPs-Cas14a based PEC for PTK7 detection. B) PAGE electropherogram verifying the SDA triggered by the complementary DNA of PTK7 aptamer (M1: 20 bp marker; a: T-DNA; b: cDNA; c: cDNA + T-DNA; d: cDNA + T-DNA + KF; e: cDNA + T-DNA + Nt; f/g/h: 0.5/2/5 µM cDNA + T-DNA + KF + Nt; M2: 50 bp marker). C) I-T bar curves corresponding to different concentrations of PTK7. D) Standard curve of UCNPs-Cas14a based PEC for the detection of T2. E) Specificity of UCNPs-Cas14a based PEC sensing. F) Stability of UCNPs-Cas14a based PEC sensing to detect PTK7