Fig. 3

Physicochemical properties of H-LPAE/DNA polyplexes. (a) DNA binding affinity measured by PicoGreen assays (n = 3); (b) and (c) Size (b) and Zeta potential (c) of polyplexes (n = 3); (d) TEM images of two H-LPAEs/DNA polyplexes at a w/w of 20:1. The scale bar is 500 nm. (e) Representative cellular uptake images of HeLa cells after incubation with AF647-labeled polyplexes, Scale bars, 50 μm. (f) Representative fluorescence colocalization images of H-LPAE_{B4−S5−PET4A}/ AF647-DNA polyplexes and acidic endosomes stained with Lyso Tracker Green. Scale bars, 50 μm