Fig. 4
Optimized H-LPAE/DNA polyplexes show great superiority in gene transfection for various cells. (a) Representative fluorescence images of HeLa cells after transfection with various H-LPAEs/DNA polyplexes. Scale bars, 100 μm; (b) Histogram distribution of GFP-positive populations in HeLa cells after transfection with various H-LPAEs/DNA polyplexes; (c) The percentage of GFP-positive cell and mean fluorescence intensity of HeLa cells after transfection with various H-LPAEs/DNA polyplexes, compared to jetPEI (*, p < 0.05) and Lipo3000 (#, p < 0.05) (n = 3, Student’s t test); (d) Representative fluorescence images of SW1353 cells after transfection with various H-LPAEs/DNA polyplexes. Scale bars, 100 μm; (e) Histogram distribution of GFP-positive populations in SW1353 cells after transfection with various H-LPAEs/DNA polyplexes; (f) The percentage of GFP-positive cell and mean fluorescence intensity of SW1353 cells after transfection with various H-LPAEs/DNA polyplexes. For the percentage of GFP-positive cell, compared to jetPEI (*, p < 0.05), and Lipo3000 (#, p < 0.05); For the mean fluorescence intensity, compared to jetPEI (*, p < 0.05), and Lipo3000 (#, p < 0.05). Data is represented as mean ± SD (n = 3, Student’s t test)