Fig. 6

Optimized H-LPAEs showed high TRAIL gene transfection efficiency to induce apoptosis in various cancer cells. (a) Representative microscopy images and fluorescence images of HepG2 cells after transfection with various H-LPAEs/DNA polyplexes. Scale bars, 100 μm; (b) Flow cytometry analysis of apoptosis efficiency in HepG2 cells after transfection with various H-LPAEs/DNA polyplexes; (c) Quantification of the apoptosis efficiency corresponding to the (b) (*p < 0.05 compared to Lipo3000, n = 3); (d) Representative microscopy images and fluorescence images of HeLa cells after transfection with various H-LPAEs/DNA polyplexes. Scale bars, 100 μm; (e) Flow cytometry analysis of apoptosis efficiency in HeLa cells after transfection with various H-LPAEs/DNA polyplexes; (f) Quantification of the apoptosis efficiency corresponding to the (e). Data is represented as mean ± SD, *p < 0.05 compared to Lipo3000 (n = 3). BF represents bright field