Fig. 4

LyOK-432 in combination with DOX activated cGAS/STING pathway in DCs (n = 3). A In vitro illustration of study schedule for co-culture of Hep 1–6 cells, imDC, and CD8⁺ T cells after management of iRFA and OD, O, D, or control. (B–E) Quantitative analysis of the surface makers of mature DCs (CD80⁺ CD86⁺ DC). The expression level of CD80 and CD86 was highest in ROD/OD group. F The cytotoxicity of CD8⁺ T cells induced in vitro against Hep1-6 cells in the four groups as revealed by CCK-8 assay. The cancer cells were significantly suppressed after OD management. G Western blotting showed that the protein expression of phosphorylated STING and IRF3 was higher in OD group than in the other groups. H, I The IFN-β expression measured by qPCR and ELISA after different in vitro treatments. J, K The IFN-β expression in tumor tissues measured by qPCR and ELISA after different in vivo treatments (n = 5). cGAS, cyclic guanosine phospho-adenosine synthase; STING, stimulator of interferon genes; MACS, magnetic cell sorting. *p < 0.05; **p < 0.01; ***p < 0.001