Fig. 4

NIR-II imaging and FGS of orthotopic CRC with mesenteric lymphatic metastases. a BLI of orthotopic CRC with mesenteric lymphatic metastases. b The representative NIR-II in vivo imaging of orthotopic CRC with mesenteric lymphatic metastases at different time points post-injected with FE-2PEG via tail vein. Scale bar: 1 cm. c Quantitative analysis of fluorescence intensity of the abdominal ROIs at different time points in (b) (n = 3, data are shown as mean ± SD). d White light and NIR-II imaging of exposed tumors after laparotomy in vivo. Scale bar: 5 mm. e White light and NIR-II imaging of the orthotopic tumor (P), mesenteric lymphatic metastases (M1 ~ M14), and abdominal metastases (A1 ~ A3) ex vivo. Scale bar: 1 cm. f White light and NIR-II imaging of isolated orthotopic tumor (P), mesenteric lymphatic metastases (M1 ~ M14), and abdominal metastases (A1 ~ A3) ex vivo. Scale bar: 1 cm. g TNR of the primary lesion (P), mesenteric lymphatic metastases (M1 ~ M14), and abdominal metastases (A1 ~ A3). h Plot profiles of the representative mesenteric lymphatic metastasis (Line 1) in (e). i H&E staining of the primary tumor (P), mesenteric lymphatic metastases (M1 ~ M14), and abdominal metastases (A1 ~ A3). Scale bar: 100 μm