Fig. 5

NIR-II microscopic imaging of orthotopic CRC with lymphatic metastases. a White light and NIR-II in vivo imaging of orthotopic CRC with lymphatic metastases post-injection of FE-2PEG via the tail vein at 24 h. Scale bar: 1 cm. b NIR-II imaging of orthotopic tumor and mesenteric lymphatic metastases ex vivo. Scale bar: 1 cm. c White light and NIR-II imaging of the isolated orthotopic tumor, LNs, and feces ex vivo. Scale bar: 1 cm. d The H&E staining, white light microscopic, NIR-II microscopic, and the merged imaging of the orthotopic tumor. Scale bar: 100 μm (10x); Scale bar: 50 μm (20x). e The H&E staining, white light microscopic, NIR-II microscopic, and the merged imaging of the lymph node 1 (LN 1). Scale bar: 100 μm (10x); Scale bar: 50 μm (20x). f The H&E staining, white light microscopic, NIR-II microscopic, and the merged imaging of the lymph node 2 (LN 2). Scale bar: 100 μm (10x); Scale bar: 50 μm (20x). g The H&E staining, white light microscopic, NIR-II microscopic, and the merged imaging of the lymph node 3 (LN 3). Scale bar: 100 μm (10x); Scale bar: 50 μm (20x). h The H&E staining, white light microscopic, NIR-II microscopic, and the merged imaging of the normal intestinal canal. Scale bar: 100 μm (10x); Scale bar: 50 μm (20x)