Fig. 3
High-specificity cancer therapy efficacy in vitro. a Schematic illustration for R-PtMn-1 involved in the ferroptosis process. Confocal images (b) and corresponding fluorescent intensity (c) of DCHF-DA-stained 4T1 cells pre-treated Nr-PtMn-1 or R-PtMn-1. Confocal images (d) and corresponding fluorescent intensity (e) of DCHF-DA-stained 4T1 cells pre-treated with R-PtMn-1 with different Mn concentrations. Confocal images (f) and corresponding fluorescent intensity (g) of DCHF-DA-stained 4T1 cells pre-treated with R-PtMn-1 for different incubation times. Confocal images (h) and corresponding fluorescent intensity (i) of liperfluo-stained 4T1 cells pre-treated with R-PtMn-1 with different Mn concentrations. Confocal images (j) and corresponding fluorescent intensity (k) of liperfluo-stained 4T1 cells pre-treated with Nr-PtMn-1 or R-PtMn-1. Confocal images (l) and corresponding fluorescent intensity (m) of liperfluo-stained 4T1 cells pre-treated with R-PtMn-1 for different incubation times. Confocal images (n) and the ratio of green/ red fluorescent intensity (o) of JC-1 stained 4T1 cells pretreated with Nr-PtMn-1 or R-PtMn-1. p Intracellular MDA level for 4T1 cells treated with Nr-PtMn-1 or R-PtMn-1. q Intracellular GSH level for 4T1 cells treated with Nr-PtMn-1 or R-PtMn-1. r Intracellular GSH level for 4T1 cells treated with R-PtMn-1 with different Mn concentrations. s Intracellular GSH level for 4T1 cells treated with R-PtMn-1 for different incubation times. t–u The expression of Western blotting assay for 4T1 cells treated with Nr-PtMn-1 or R-PtMn-1. t GPX4 protein. u ACSL-4 and BID protein. v The relative cellular viability of 4T1 cells treated with various concentrations of R-PtMn-1 for 24 h. w The relative cellular viability of 4T1 cells treated with R-PtMn-1 (240 μg/mL) at different times. x The relative cellular viability of CT26 cells treated with various concentrations of R-PtMn-1 for 24 h. y The relative cellular viability of 4T1 cells treated with various concentrations of Nr-PtMn-1 for 24 h. The statistical analysis was performed in contrast to the control group (*p < 0.05, **p < 0.01, ***p < 0.001, t-test)