Fig. 2
From: Probing milk extracellular vesicles for intestinal delivery of RNA therapies
Apical-out culture of human (biopsy-derived) colon intestinal epithelial organoids (IEOs) and transport of milk extracellular vesicles (mEVs). A Brightfield image of IEOs cultured in Matrigel (basal-out) and PET transwells inserts (apical-out), and the schematic for development of apical-out IEOs from basal-out polarity. B Depicted schematic of basal-out and apical-out IEOs. C Confocal immunofluorescent staining images of IEOs with apical-out and basal-out polarity. D Confocal immunofluorescent staining images of mEVs transporting across apical-out IEOs after incubation for 4 h. Nucleus in blue (DAPI), apical zonula occludens (ZO-1) tight junction protein in green, and mEVs in red. Schematics were drawn by Figdraw