Fig. 3

M-FNM induces GSDMD-mediated pyroptosis in CAL27 cells. (A) Schematic illustration of M-FNM Trigger pyroptosis. (B) Representative bright-field microscopy image of CAL27 cells with M-FNM treatment. (C) CLSM fluorescence images of ROS detected by the DCFH-DA after cells were incubated with M-FNM for 1 h. (D) Corresponding semi-quantitative analysis of DCFH-DA. (E) Western blot analysis of N-GSDMD ASC, IL-1β, and cleaved Caspase-1 in CAL27 cells were co-cultured with M-FNM, protein collection after vacuolar lysis of cells. (F) Release of LDH from the supernatant of CAL27 cells incubated with M-FNM for 24 h. (G) CLSM fluorescence images of ASC (yellow arrows) after cells were co-cultured with M-FNM. (H) CLSM fluorescence images of N-GSDMD in tumor after M-FNM treatment. (I) CLSM fluorescence images of MSCs after M-FNM treatment. (M-FNM: 300 µg/mL). Compared to control, *P < 0.05, **P < 0.01, and ***P < 0.001. The mean values and standard deviations represent the average of biological triplicates