Fig. 4
From: Mannose-doped metal-organic frameworks induce tumor cell pyroptosis via the PERK pathway
M-FNM activates the PERK pathway to induce pyroptosis. (A) Mechanism illustration of M-FNM mediates pyroptosis. (B) CLSM images of calcium ions in the cytoplasm after M-FNM treatment. (C) Corresponding semi-quantitative analysis of Fluo-4am. (D) CLSM fluorescence images of cleaved Caspase-1 in CAL27 cells after M-FNM treatment. (E) IL-1β release in supernatant after M-FNM treatment. (F) Western blot analysis PERK pathway in CAL27 cells after M-FNM treatment. (G) CLSM images of Annexin V/PI toward CAL27 cells. (H) Western blot analysis of N-GSDMD, cleaved Caspase-1, and IL-1β in CAL27 cells. (4-PBA: 5mM; M-FNM: 300 µg/mL). Compare to control, *P < 0.05, **P < 0.01, and ***P < 0.001. The mean values and standard deviations represent the average of biological triplicates