Fig. 2

The isolation process of bovine milk-derived exosome. ①: Dairy cow; ②: Raw milk; ③: Divide milk into EP tubes; ④: Centrifuge at 12,000×g, 4 ℃ for 30 min to remove the remaining fat and cell debris; ⑤: Skimmed milk; ⑥: Skimmed milk was transferred into an ultracentrifugation tubes; ⑦: Centrifuge at 150,000×g, 4 ℃ for 2 h; ⑧: The exosome pellet was collected and transferred into a low binding tube and resuspended in phosphate buffer solution (PBS) to 500 µL; ⑨: the suspension sample was loaded on a qEV original 35 nm SEC column; ⑩ After 3 mL of void volume, 4 fractions (A, B, C, D) of each 500 µL were immediately collected