Fig. 1From: PLGA nanoparticles engineering extracellular vesicles from human umbilical cord mesenchymal stem cells ameliorates polyethylene particles induced periprosthetic osteolysisExtraction and characterization of exosomes from human umbilical cord mesenchymal stem cells. (A) Flow cytometry analysis of the typical surface markers in HucMSCs. Blue curves: the isotype controls; Red curves: the test samples. (B) TEM images of Exos. Scale bar, 100 nm. (C) Size distribution (PDI = 0.14) and zeta potential of Exos were measured by dynamic light scattering (n = 3). (D) Typical surface markers expressed on HucMSCs and Exos were determined by Western blot analysis (Calnexin: 90 kDa, CD9: 25 kDa, CD63: 26 kDa, TSG101: 45 kDa)Back to article page