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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: PLGA nanoparticles engineering extracellular vesicles from human umbilical cord mesenchymal stem cells ameliorates polyethylene particles induced periprosthetic osteolysis

Fig. 4

PLGA-Exos inhibited Osteoclast Formation in vitro. (A) Representative immunofluorescence images showing the internalization of DII-labeled Exos (red) by RAW264.7 cells and the cell nuclei of RAW264.7 cells were stained with DAPI (blue). Scale bar: 10 μm. (B) The production of ROS (green) was assayed using a fluorescence microscope. Scale bar: 200 μm. (C-E) The number of TRAP+ multinucleated (> 3 nuclei) osteoclasts and the TRAP+ cell areas were determined. (F, G) The IL-6 and TNF-α levels in the medium (RAW264.7 cells) were determined with ELISA. (n = 3 samples per group, ANOVA, *P < 0.05, ** P < 0.01, *** P < 0.001)

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Journal of Nanobiotechnology

ISSN: 1477-3155

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