Fig. 7

Histological staining and histomorphometry analysis of calvaria sections. (A) Representative images of H&E staining. Scale bar, 200 μm. (B) Representative images of TRAP staining and the corresponding areas were enlarged. Scale bar, 200 µ m (left), 50 µ m (right). (C-E) Quantitative analyses of eroded surface area, number of TRAP⁺ osteoclasts, and OcS/BS were calculated. (n = 3 samples per group, ANOVA, *P < 0.05, ** P < 0.01, *** P < 0.001)