Fig. 2

Confirmation of the synthesis of the cAb-beads. (a) CLSM images of Fe₃O₄-Au composites and cAb-beads before and after the incubation with red fluorescent rIgG-PE. (b) CLSM images of green fluorescent PKH67-labeled LC3⁺ EVs, cAb-beads, PKH67-labeled LC3⁺ EVs after incubating with cAb-beads, and PKH67-labeled LC3⁺ EVs after incubating with LC3 cAb and cAb-beads successively. (c) The absorbance at 450 nm of TMB from the HBcAg ELISA kit after the test with PBS, cAb-beads, the mixture of cAb-beads and HBcAg, and HBcAg. ^***p < 0.001, ns: not significant. (d) CLSM images of cAb-beads after the incubation with red fluorescent mIgG-594, and cAb-beads after the incubation with the mixture of mIgG-594 and PKH67-labeled LC3⁺ EVs. (e) Western blot analysis of LC3 proteins (LC3 I and LC3 II) lysized from LC3⁺ EVs, and the supernatant and precipitation after the magnetic separation of LC3⁺ EVs by using cAb-beads. (rIgG-PE: PE-conjugated goat anti-rabbit IgG, mIgG-594: Alexa Fluor 594-conjugated goat anti-mouse IgG)