Fig. 2
CeO2@RSV manipulates the infiltration of macrophages and reduces the inflammatory response. A Representative TEM images of macrophages incubated with CeO2@RSV (50 μg/mL) for 48 h. Scale bar: 10 µm. The arrowhead indicates intracellular NPs. B The diagram describes the effect of CeO2@RSV on the repolarization of macrophages from M1 to M2. mRNA expression levels of iNOS (C), IL-1β (D) and TNF-α (E) were determined by RT-PCR in M1 macrophages treated with NPs. mRNA expression levels of TGF-β (F) and IL-10 (G) in different experimental treatment groups. H Immunofluorescence results of iNOS and CD206 in different experimental treatment groups. Scale bar: 50 μm. I ELISA results of IL-1β and IL-10 levels in the supernatants of the different treatment groups. J Flow cytometry analysis of specific markers of M1 (CD86) and M2 (CD206) macrophages in different experimental treatment groups. K Representative histograms and MFIs of DCF fluorescence in M1 macrophages treated with NPs. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001