Fig. 2
From: TROP2-directed nanobody-drug conjugate elicited potent antitumor effect in pancreatic cancer
Structure and binding affinity of HuNbTROP2-HSA-MMAE. A Schematic structure of HuNbTROP2-HSA-MMAE. B The SDS-PAGE analysis of HuNbTROP2-HSA and HuNbTROP2-HSA-MMAE with the addition of the reducing agent TCEP and/or the oxidizing agent CuSO4. M represents marker. C The affinity of HuNbTROP2-HSA and HuNbTROP2-HSA-MMAE for TROP2 proteins was determined by ELISA. D Flow cytometry assessed the binding activities of HuNbTROP2-HSA and HuNbTROP2-HSA-MMAE to TROP2-positive pancreatic cancer cells in the presence and absence of 10% human serum