Fig. 3
From: TROP2-directed nanobody-drug conjugate elicited potent antitumor effect in pancreatic cancer
Endocytosis and in vitro antitumor activity of HuNbTROP2-HSA-MMAE. A The process of HuNbTROP2-HSA-MMAE internalization into BxPC-3 cells. Blue: nuclei stained by Hoechst33342; green: HuNbTROP2-HSA-MMAE labeled with Alexa Fluor™ 488. B HuNbTROP2-HSA-MMAE underwent lysosomal degradation after internalization. Blue: nuclei; green: HuNbTROP2-HSA-MMAE; red: lysosomes. C The expression of TROP2 on the surface of three human pancreatic cell lines was determined by flow cytometry and the cytotoxic effects of HuNbTROP2-HSA-MMAE treatment for 2 or 3 days on these cancer cells were evaluated by MTT assay