Fig. 4
From: TROP2-directed nanobody-drug conjugate elicited potent antitumor effect in pancreatic cancer
Cell apoptosis induced by HuNbTROP2-HSA-MMAE. A The effect of HuNbTROP2-HSA-MMAE on apoptosis of BxPC-3 and PK-59 cells was detected by Annexin V-FITC/PI staining. Annexin V+PI− (Q4) represents early apoptotic cells and Annexin V+PI+ (Q2) represents late apoptotic cells. B A statistical analysis of three replicate experiments of (A). The proportion of apoptotic cells is the sum of Q2 and Q4. C Western Blot demonstrated the expressions of apoptosis-related proteins in pancreatic cancer cells after 72 h of treatment of HuNbTROP2-HSA-MMAE