Fig. 2

MiR-184-3p enriched in T-exo was delivered to macrophages to promote tumor progression. a Venn diagram showed the overlap of miRNAs in two batches of T-exo detected by miRNA-seq. b Heatmap of the top 10 highly expressed miRNAs in (a). c Volcano of differentially expressed miRNAs in macrophages treated with or without T-exo detected by miRNA-seq. d Heatmap of the up-regulated miRNAs with high and middle expression in (c). e The expression of miR-184-3p in macrophages and T-exo detected by qRT-PCR (n = 3). CD206 levels (f) and secreted IL-10 (g) of macrophages transfected with negative control (N.C.) or miR-184-3p mimics (n = 3). h The proliferation of 4T1 cells cultured with MCM from macrophages treated as described above assessed by MTT assay (n = 3). Representative images of transwell migration assay (i) and the relative migration cell number (j) of 4T1 cells co-cultured with macrophages treated as described above (n = 3). Tumor volume changes (k), image of tumors (l) and tumor weight (m) of each group (n = 5). n The expression of Cd206 in tumor tissues detected by qRT-PCR (n = 3). Data were expressed as mean ± SD (**p < 0.01, ***p < 0.001)