Fig. 3

Exosomal miR-184-3p induced M2 macrophage polarization by inhibiting EGR1 expression and JNK signaling pathway. a Venn diagram showed the overlap of mRNAs in down-regulated genes in M0-T-exo detected by mRNA-seq and the predicted target genes of miR-184-3p. b Heatmap of 12 mRNAs screened from (a). c The expression of Egr1 gene in M0-T-exo and M0 macrophages detected by qRT-PCR. d The expression of EGR1 protein in M0-T-exo and M0 macrophages determined by IF staining. Cell nuclei were blue. Rac1 was red. e MFI of Rac1 in (d) calculated by ImageJ software. f The expression of Egr1 gene in macrophages transfected with N.C. or miR-184-3p mimics detected by qRT-PCR. g The expression of EGR1 protein in macrophages transfected with N.C. or miR-184-3p mimics determined by IF staining. Cell nuclei were blue. Rac1 was red. h MFI of Rac1 in (g) calculated by ImageJ software. Western blot assays for p-JNK expression in M0-T-exo (i) and macrophages transfected with miR-184-3p mimics (j). Data were expressed as mean ± SD (n = 3, ***p < 0.001)