Fig. 4

MiR-184-3p functioned as a tumor suppressor by down-regulating MAML1. a The expression of miR-184-3p in M0 macrophages, 4T1 cells and T-exo detected by qRT-PCR. b The proliferation of 4T1 cells transfected with N.C. or miR-184-3p mimics determined by MTT assay at different time points. Representative images of the wound-healing assay (c) and wound-healing percentage (d) of 4T1 cells transfected with N.C. or miR-184-3p mimics. e The expression of miR-184-3p in mammary glands and tumors of different sizes measured by qRT-PCR. The gene expression of Maml1 in 4T1 cells transfected with miR-184-3p mimics (f) or miR-184-3p inhibitors (g) determined by qRT-PCR. The protein levels of MAML1 in 4T1 cells transfected with miR-184-3p mimics (h) or miR-184-3p inhibitors (j) determined by IF staining. Cell nuclei were blue. MAML1 was red. i MFI of MAML1 in (h) calculated by ImageJ software. k MFI of MAML1 in (j) calculated by ImageJ software. Data were expressed as mean ± SD (n = 3, **p < 0.01, ***p < 0.001)