Fig. 5

HnRNPA2B1 directly mediated miR-184-3p packaging into tumor cell-derived exosomes. a The expression of miR-184-3p in macrophages co-cultured with 4T1 cells pre-treated with or without GW4869 by qRT-PCR (n = 3). b RIP analysis of miR-184-3p bound by hnRNPA2B1 by incubating 4T1 cell lysates with IgG or anti-hnRNPA2B1 (n = 3). Intracellular (c) and exosomal (d) levels of miR-184-3p in 4T1 cells transfected with N.C. or si-hnRNPA2B1 (n = 3). e The proliferation of 4T1 cells transfected with N.C. or si-hnRNPA2B1 determined by MTT assay at different time points (n = 3). The expression of miR-184-3p (f) and Cd206(g) in macrophages co-cultured with 4T1 cells transfected with N.C. or si-hnRNPA2B1 by qRT-PCR (n = 3). h ELISA for the secreted IL-10 in MCM (n = 3). Image of tumors (i), tumor volume changes (j), and tumor weight (k) of each group (n = 5). l H&E staining for livers and lungs from tumor-bearing mice. Tumor metastases were marked by yellow lines. The expression of miR-184-3p (m) and Cd206(n) in tumor tissues detected by qRT-PCR (n = 3). o ELISA for the secreted IL-10 in plasma from tumor-bearing mice (n = 3). Data were expressed as mean ± SD (*p < 0.05, **p < 0.01, ***p < 0.001)