Fig. 2

Characterization of SARS-CoV-2 nucleocapsid protein with monoclonal antibodies specific to the nucleocapsid protein. A SDS-PAGE analysis of N expression protein (upper panel); western blotting analysis of N expression protein using mouse serum (bottom panel). B Western blotting analysis of N protein using mAbs produced in this study. C Analysis of the binding affinity (Kd value) of monoclonal antibody-3A5D12 ( ) and 6F6E11 ( ). The results represent the mean ± SD of absorbance value obtained from three independent replicates. D Three-dimensional structure prediction of SARS-CoV-2 nucleocapsid protein. E Three-dimensional structure prediction of the monoclonal antibody-6F6E11. F Docking site prediction between N protein and mAb-6F6E11