Fig. 3

Characterization of the SARS-CoV-2 N protein aptamer. A Selection of aptamer after SELEX using capillary electrophoresis. B Affinity analysis of aptamer to N protein using iELISA. C Western blotting analysis of Apt #6 and Apt #8 affinity to N protein. D Analysis of the binding affinity (Kd value) of Apt #6. The results represent the mean ± SD of absorbance value obtained from three independent replicates. E Secondary structure prediction of Apt #6. F Three-dimensional structure prediction of the Apt#6 G Docking site prediction between N protein and Apt #6