Novel enzyme-linked aptamer-antibody sandwich assay and hybrid lateral flow strip for SARS-CoV-2 detection

Table 1 Analysis of uninfected and spiked nasopharyngeal samples using hybrid ELAAA and hybrid-LFS

No.^a	Spike N protein (ng/mL)	ELAAA (ng/mL)^b	CV (%)^c	Recovery (%)	Hybrid-LFS
N₁ – N₁₀	0	N.D^d			―
N₁-S	1000	990 ± 22.2	2.2	99	+
N₂-S	1000	917 ± 50.4	5.5	92	+
N₃-S	500	461 ± 20.8	4.5	92	+
N₄-S	500	487 ± 43.0	8.8	97	+
N₅-S	50	40 ± 0.4	1.0	80	+
N₆-S	50	43 ± 0.2	0.5	86	+
N₇-S	1	0.95 ± 0.09	9.5	95	+
N₈-S	1	0.96 ± 0.03	3.1	96	+
N₉-S	0.5	0.47 ± 0.02	4.3	94	+
N₁₀-S	0.5	0.44 ± 0.02	4.5	88	+
Overall			4.4	92

Each sample was analyzed in three repeats; ^aN₁ – N₁₀ corresponds to 10 volunteers, while N₁-S –N₁₀-S indicates their respective spiked samples; ^bThe ELAAA results are presented as mean ± SD; ^cThe Coefficient of Variation (CV) is calculated using the following formula: \(\frac{SD}{Mean}\times 100\%\); ^dN.D, the measured N protein concentrations were all below the detection limit of the hybrid ELAAA

ISSN: 1477-3155