Fig. 5
From: Fe-doped carbon dots: a novel biocompatible nanoplatform for multi-level cancer therapy
The ability of Fe-CDs to modulate macrophage polarization. a Prussian blue staining of Fe in macrophages incubated with Fe-CDs for different time. b Cell viability of macrophages incubated with Fe-CDs and FeG by MTT assay. c qPCR assay (left) and ELISA assay (right) of IL-10 expression level in macrophages with (right) and without (left) tumor cell medium, in the presence of Fe-CDs or FeG. d Western blot of Arg-1 expression levels in macrophages incubated with (right) or without (left) tumor cell medium, in the presence (+) or absence (−) of Fe-CDs and FeG. e Immunofluorescence images of IL-10 in macrophages with or without tumor cell medium, in the presence or absence of Fe-CDs and FeG. f Western blot of expression levels of M2-type macrophage-related proteins in macrophages treated with tumor cell medium in the presence of Fe-CDs at different incubation time. g Immunohistochemical staining of M2-type macrophage marker in cancerous tissues treated with PBS (left) and Fe-CDs (right). h Schematic representation of the ability of Fe-CDs in modulating macrophage polarization (Created with BioRender.com). (ns: not significant, *P < 0.01, **P < 0.001, ***P < 0.0001, n = 6)