Fig. 3
In vivo antitumor efficacy in a murine colorectal cancer model. The MC38 tumor bearing-mice were intravenously injected the formulations at SN38-equivalent dose of 5 mg/kg and then treated without or with US irradiation (0.8 W/cm2, 3 MHz, 50% of duty cycle, 5 min) at 6 h after intravenous injection. A The experimental timeline and tumor treatment schedule in murine colorectal tumor model. B The changes in the tumor volume during the treatment. C The changes in body weight in each group. D The inhibition rates of tumor growth (IRT) and tumor weights. E The photograph of resected MC38 tumors in each group. F Liver-related serum biochemical analysis of ALT and AST and the kidney function indicators of BUN and CREA. G Representative images of the Ki67, TUNEL, and H&E staining of the tumor slices. Scale bar = 50 μm (Ki67 and TUNEL staining) or 100 μm (H&E staining). Data were expressed as the mean ± SD (n = 5, ***p < 0.001, ****p < 0.0001)