Fig. 4
In vivo antitumor efficacy and side-effects evaluation of the liposome in hepatocellular carcinoma. The Huh7 tumor-bearing mice were intravenously injected with the formulations at an SN38-equivalent dose of 5 mg/kg and treated with US irradiation (0.8 W/cm2, 3 MHz, 50% of duty cycle, 5 min) at 6 h after intravenous injection in each group. The treatment was performed every 3 days for a total of 5 times, as indicated by red arrows. A The experimental timeline and tumor treatment schedule in this study. B The changes in the tumor volume during the treatment. C The inhibition rates of tumor growth (IRT) and tumor weights. D The changes in body weight in each group. E–K The blood cells and components of leukocytes, blood platelets, erythrocytes, neutrophils, monocytes, lymphocytes, and hemoglobin were evaluated in each peripheral blood sample. L Liver-related serum biochemical analysis of ALT and AST, and the kidney function indicators of BUN and CREA. M Representative images of the H&E staining and TUNEL assay staining of intestinal cross-sections in each group. Scale bar = 100 μm. Data were expressed as the mean ± SD (n = 5, ***p < 0.001, ****p < 0.0001)