Fig. 7
PDGF-BB promotes MSC migration and protects MSCs against apoptosis. (A-B). The wound healing assay illustrated MSC migration 24 h after PDGF-BB treatment at different concentrations. Bar, 200 μm. N = 6/group. *p < 0.01 vs. vehicle; # p < 0.01 vs. 10 ng/ml; & p < 0.01 vs. 20 ng/ml. (C-D). Cell migration was detected by Transwell assay after 24 h of treatment with or without PDGF-BB. Bar, 100 μm. N = 6/group. *p < 0.05 vs. vehicle. (E-G). MSC apoptosis was assessed by Annexin V/PI staining followed by flow cytometry analysis. H2O2, 200 µM, 6 h. Values are the mean ± SEM. Significant differences were determined by using one-way ANOVA. N = 6/group. *p < 0.01 vs. vehicle; # p < 0.01 vs. PDGF-BB; & p < 0.01 vs. H2O2-vehicle