Fig. 4

A Schematic representation of the pharmacokinetic study. To measure rtPA plasmatic activity, blood samples were extracted before (basal) and 1, 5, 15 and 40 min after the treatment administration. B Variation of plasmatic tPA activity during a period of 40 min after treatment administration of groups treated with vehicle, rtPA (1 mg/kg as bolus), fresh and lyophilized CSM@rtPA (1 mg/kg as bolus). Data are represented as mean ± SEM (n = 6 per group of treatment). Statistical analysis was assessed by Kruskal–Wallis test followed by Dunn’s multiple test comparing all the groups at the same timepoint (*P < 0.05; **P < 0.01). C Schematic representation of the bleeding assay. After the administration of the treatments (vehicle, 1 mg/kg rtPA, 1 mg/kg CSM@rtPA and 1 mg/kg CSM@rtPA/L) the tail was cut off. D Percentage of the animals in which re-bleeding occurred and E the bleeding time was quantified. Data represent mean ± SEM (n = 5 per group of treatment). Statistical analysis was assessed by a one-way ANOVA followed by Tukey’s multiple comparison test comparing all the groups (*P < 0.05)