Fig. 3

NanoFVax elicited BMDCs maturation in vitro. CD80 + (A), CD86 + (B), and MHC-II + (C) expression on mDCs determined by flow cytometry after co-incubation with different stimulants for 48 h. The bar graphs show the statistics for CD80 + (A), CD86 + (B), and MHC-II (C). Comparison of cytokines and chemokines in BMDC supernatants of each group. Culture supernatants were analyzed for levels of the Th1 cytokine, IL-2 (D), the Th2 cytokine, IL-10 (E) (n = 3), and F IL-21, CD40L, and the chemokines CXCL10, CCL5 as measured by ELISA (n = 4). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 by Student’s t-test