Fig. 4

Confocal images of cellular uptake and in vivo bio-distribution of NanoFVax. A Visualization of cellular uptake of fluorescein isothiocyanate (FITC)-labeled NanoFVax-treated DC cells after 24 h. Numbers indicate relative intensity as quantified by Image J analysis. DAPI staining (blue) was used to visualize nuclei. Actin cytoskeleton was visualized with phalloidin-iFluor 594. Free FITC was used as a control. Numbers indicate relative intensity as quantified by image J analysis. B Fluorescence imaging of mice of the different injection groups. Representative images of one mouse from the two mice per group are shown. C Ex vivo fluorescence imaging of major organs 48 h after subcutaneous injection. (The first column is the lungs, kidneys, spleen, and heart from top to bottom; the second column is the lymph nodes)