Fig. 5
Diagram illustrating the experimental schedule. A BALB/C mice were immunized according to the immunization procedure. Five mice per group were vaccinated with different vaccines on days 0, 14, and 28. Serum was collected periodically. Three mice were euthanized on days 42. B Serum IgG antibody titers were determined by the serial dilution method, with specific IgG titers calculated for each sample and plotted as time course curves expressed as the reciprocal of the endpoint serum dilution. Serum IgG titers were transformed to log10. Thus, all concentrations were log10 transformed prior to statistical analysis. (Mean log10 IgG titers = 5.1). C Serum was collected from mice and Ig (IgG1, IgG2a, IgG2b, and IgG2c, IgA, IgM,) levels were measured on days 42 as well as the IgG1/IgG2a ratio (D). E Body weight changes of treated mice following intramuscular injection (n = 5). F Serum cytokines (IL-2, IFN-γ, IL-4, TNF-α, IL-10, and IL-12) were assayed for each group 42 days after immunization. Data are the mean of three independent experiments. Mean values and error bars are defined as means and S.D., respectively. Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.0001; ****p < 0.00001